Journal: Nature Communications
Article Title: Targeting CD37 promotes macrophage-dependent phagocytosis of multiple cancer cell types and facilitates tumor clearance in mice
doi: 10.1038/s41467-025-61348-2
Figure Lengend Snippet: A Illustration of the ribosome profiling in phagocytes, macrophages, and cancer cells. Peripheral blood monocytes were isolated from healthy donors and differentiated into macrophages. The macrophages were labeled with CellTracker Blue and co-cultured with CFSE-labeled cancer cells for 2 h. Subsequently, flow cytometry was employed to sort the macrophages, phagocytes, and cancer cells as indicated. Cells were then subjected to ribosome profiling analysis. A total of 100 healthy donors were enrolled. B The volcano plot illustrates the identification of dysregulated genes, with phagocyte-specific genes determined by comparing phagocytes to MDA-MB-231 cancer cells (FPKM = 0 in MDA-MB-231 cells). Dysregulated genes were subsequently identified by comparing these phagocyte-specific genes to those in control macrophages. Differentially expressed genes (DEGs) were defined as those meeting the criteria of p < 0.05 and fold change > 2. FC fold change, FDR false detecting rate. C GO analysis of the dysregulated genes, dysregulated genes were identified, and Gene Ontology Analysis was applied to analyze the enriched pathways of dysregulated genes. D The detail of the dysregulated genes enriched pathways, gene ontology analysis was applied to analyze the dys-enriched pathways, the detail of the pathway, including the q value and the number of dysregulated genes, was shown. E The rank of the dysregulated receptor and transmembrane protein, PD1, Siglec-10, and CD37, was indicated, X -axis, the rank of the genes, Y -axis, the log 2 FC, FC fold change. F The rank of the dysregulated receptor and transmembrane protein (RPKM > 0 in phagocytes), X -axis, the rank of the genes, Y -axis, the log 2 FC, FC fold change.
Article Snippet: Macrophages were then labeled with CellTracker CMF2HC (Thermo Fisher Scientific, Catalog # C12881 ), and breast cancer cells MDA-MB-231 were labeled with CellTrace CFSE (Thermo Fisher Scientific, Catalog # C34554 ).
Techniques: Isolation, Labeling, Cell Culture, Flow Cytometry, Control
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